N4142
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 | Immunofluorescence Primary neuronal culture of rat amygdala. Nuclei stained blue with DAPI (Cat. No. D9542), neuron cluster (lower left) ensheathed by gail processes stained red with Rabbit Anti-GFAP (Cat. No. G9269) and Goat Anti-Rabbit IgG-TRITC (Cat. No. T6778) and positive neuronal fibers stained green with Rabbit Anti-Neurofilament 200 (Cat. No. N4142) and Goat Anti-Rabbit IgG-FITC (Cat. No. F9887). From R. Gabr, West Virginia Univ., Dept of Pharmacology & Toxicology, Morgantown, WV. |
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N4142
Sigma
Anti-Neurofilament 200 antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
| Synonym: | AntiNeurofilament heavy chain |
| MDL number: | MFCD02263444 |
Description
| Immunogen | neurofilament 200 from bovine spinal cord. |
| Specificity | Shows a wide species cross-reactivity. Reacts with neurofilaments in the central and peripheral nervous system and localizes the neurofilament polypeptide. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | IgG fraction of antiserum |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 200 kDa |
| species reactivity | wide range, bovine |
| application(s) | immunoblotting: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using animal cerebellum sections | |
| microarray: suitable | |
| Gene Information | human ... NEFH(4744) |
| rat ... Nefh(24587) | |
| mouse ... Nefh(380684) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
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References
| reference | Basso, A.S., et al., Reversal of axonal loss and disability in a mouse model of progressive multiple sclerosis J. Clin. Invest. 118, 1532-1543, (2008) Abstract |
| Eilers, A., et al., Distinct requirements for TrkB and TrkC signaling in target innervation by sensory neurons Genes Dev. 16, 633-645, (2002) Abstract | |
| Lin, G., et al., Regeneration of neural crest derivatives in the Xenopus tadpole tail BMC Dev. Biol. 7, 56, (2007) Abstract |
